APPLICATION ID 1149

Nadolol (four isomers)

COMPOUND IDENTIFICATION

Compound Name Nadolol (four isomers)
Nadolol (four isomers)

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® AD-H

250 x 4.6mm / 5µm

Mobile Phase Composition

acetonitrile / 1-butanol / ethanolamine = 95 / 5 / 0.1

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

1

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

7.2

1.4

2

10.3

2.4

3

11.6

2.9

4

36.8

11.3

Alpha Value

Resolution

1 - 2

1.7

5.9

2 - 3

1.2

1.9

3 - 4

3.9

17

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