ID
1543

Tramadol

COMPOUND IDENTIFICATION

Molecular Weight
263.374
Formula
C16H25O2N
Property
Basic
Tramadol

Compound name

Tramadol

CHROMATOGRAM

Tramadol

ANALYTICAL REPORT

Column Trade Name
(Column size / Particle size)
CHIRALPAK® AD-RH
(4.6 x 150 mm 5 µm)
Chromatographic Mode Reverse Phase
Mobile Phase Composition 10mM ammonium bicarbonate aq. pH8.5 / acetonitrile = 65 / 35
Flow rate (mL/min) 1 Temperature (℃) 40
Injection Volume (µL) 0.005 (mg) Sample Concentration
Detection UV-VIS 220 nm
Elution Order
No. Rt K'
1 5.5 2.08
2 6.5 2.59
Alpha Value Resolution
1 - 2 1.25 1.52