APPLICATION ID 1662

Lamivudine (containing the enantiomeric impurity)

COMPOUND IDENTIFICATION

Compound Name Lamivudine (containing the enantiomeric impurity)
Lamivudine (containing the enantiomeric impurity)

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IC-3

250 x 4.6mm / 3µm

Mobile Phase Composition

acetonitrile / diethylamine = 100 / 0.1

Chromatographic Mode

Normal Phase

Flow rate (ml/min)

1.0

Injection amount (mg)

0.02

Temperature (°C)

25

Pressure (MPa)

Detection

UV 260 nm

Elution order

No.

Rt

K’

1

8.9

2

10.1

3

17.6

4

21.6

Alpha Value

Resolution

1 - 2

2.6

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