APPLICATION ID 19

5-Hydroxy-DL-tryptophan

COMPOUND IDENTIFICATION

Compound Name 5-Hydroxy-DL-tryptophan
5-Hydroxy-DL-tryptophan

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(+)

150 x 3mm / 3µm

Mobile Phase Composition

50mM formic acid + 25mM diethylamine in methanol / water = 80 / 20

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

D/L

No.

Rt

K’

1

3.03

0.84

2

3.58

1.18

Alpha Value

Resolution

1 - 2

1.4

2.81

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