APPLICATION ID 27

Atenolol

COMPOUND IDENTIFICATION

Compound Name Atenolol
Atenolol

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(+)

150 x 3mm / 3µm

Mobile Phase Composition

12.5mM di-n-butylamine in methanol / tetrahydrofuran / water = 49 / 49 / 2

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.0025

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

4.41

1.61

2

4.9

1.89

Alpha Value

Resolution

1 - 2

1.18

2.35

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