APPLICATION ID 299

Bupivacaine

COMPOUND IDENTIFICATION

Compound Name Bupivacaine
Bupivacaine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IA

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / chloroform / diethylamine = 75 / 25 / 0.1

Chromatographic Mode

Normal Phase
Secondary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

ELSD

Elution order

No.

Rt

K’

1

8.12

1.63

2

13.26

3.29

Alpha Value

Resolution

1 - 2

2.02

13.48

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