APPLICATION ID 41

DL-Ala-DL-Leu-Gly

COMPOUND IDENTIFICATION

Compound Name DL-Ala-DL-Leu-Gly
DL-Ala-DL-Leu-Gly

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(-)

150 x 3mm / 3µm

Mobile Phase Composition

50mM formic acid + 25mM diethylamine in methanol / tetrahydrofuran / water = 49 / 49 / 2

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.0015

Temperature (°C)

25

Pressure (MPa)

Detection

ELSD

Elution order

No.

Rt

K’

1

5.47

2.08

2

6.35

2.58

3

7.39

3.17

4

9.33

4.26

Alpha Value

Resolution

1 - 2

1.24

2.71

2 - 3

1.23

2.66

3 - 4

1.34

3.92

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