APPLICATION ID 49

DL-Amethopterin

COMPOUND IDENTIFICATION

Compound Name DL-Amethopterin
DL-Amethopterin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(+)

150 x 3mm / 3µm

Mobile Phase Composition

50mM formic acid + 25mM diethylamine in methanol / water = 98 / 2

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.005

Temperature (°C)

45

Pressure (MPa)

Detection

UV 254 nm

Elution order

D/L

No.

Rt

K’

1

5.31

2.32

2

7.57

3.74

Alpha Value

Resolution

1 - 2

1.61

6.29

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