APPLICATION ID 58

DL-Histidine

COMPOUND IDENTIFICATION

Compound Name DL-Histidine
DL-Histidine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(-)

150 x 3mm / 3µm

Mobile Phase Composition

50mM formic acid + 25mM diethylamine in methanol / tetrahydrofuran / water = 49 / 49 / 2

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

ELSD

Elution order

D/L

No.

Rt

K’

1

5.75

2.24

2

7.11

3.01

Alpha Value

Resolution

1 - 2

1.34

3.17

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