ID
58

DL-Histidine

COMPOUND IDENTIFICATION

Molecular Weight
155.154
Formula
C6H9N3O2
Property
Amphoteric
DL-Histidine

Compound name

DL-Histidine

CHROMATOGRAM

DL-Histidine

ANALYTICAL REPORT

Column Trade Name
(Column size / Particle size)
CHIRALPAK® ZWIX(-)
(3 x 150 mm 3 µm)
Chromatographic Mode Polar Phase
Mobile Phase Composition 50mM formic acid + 25mM diethylamine in methanol / tetrahydrofuran / water = 49 / 49 / 2
Flow rate (mL/min) 0.5 Temperature (℃) 25
Injection Volume (µL) 0.005 (mg) Sample Concentration
Detection ELSD
Elution Order D/L
No. Rt K'
1 5.8 2.24
2 7.1 3.01
Alpha Value Resolution
1 - 2 1.34 3.17