APPLICATION ID 59

DL-Homocysteine

COMPOUND IDENTIFICATION

Compound Name DL-Homocysteine
DL-Homocysteine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(+)

150 x 3mm / 3µm

Mobile Phase Composition

50mM formic acid + 25mM diethylamine in methanol / acetonitrile / water = 49 / 49 / 2

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.0015

Temperature (°C)

10

Pressure (MPa)

Detection

ELSD

Elution order

No.

Rt

K’

1

6.32

2.47

2

7.15

2.93

Alpha Value

Resolution

1 - 2

1.18

2.59

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