APPLICATION ID 67

DL-Leu-DL-Val

COMPOUND IDENTIFICATION

Compound Name DL-Leu-DL-Val
DL-Leu-DL-Val

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(+)

150 x 3mm / 3µm

Mobile Phase Composition

50mM formic acid + 25mM diethylamine in methanol / tetrahydrofuran / water = 49 / 49 / 2

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.0025

Temperature (°C)

25

Pressure (MPa)

8.7

Detection

ELSD

Elution order

No.

Rt

K’

1

4.86

1.72

2

5.63

2.15

3

6.26

2.5

4

8.65

3.84

Alpha Value

Resolution

1 - 2

1.25

3.27

2 - 3

1.17

2.27

3 - 4

1.53

6.81

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