COMPOUND IDENTIFICATION
Compound name
- Nadolol (four isomers)
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® AD-H (4.6 x 250 mm 5 µm) |
|---|---|
| Chromatographic Mode | Polar Phase |
| Mobile Phase Composition | acetonitrile / 2-propanol / ethanolamine = 95 / 5 / 0.1 |
| Flow rate (mL/min) | 1 | Temperature (℃) | 25 |
|---|---|---|---|
| Injection Volume (µL) | 0.01 (mg) | Sample Concentration | |
| Detection | UV-VIS 230 nm | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 6.7 | 1.20 |
| 2 | 9.6 | 2.20 |
| 3 | 12.1 | 3.10 |
| 4 | 45.5 | 14.10 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.80 | 5.60 |
| 2 - 3 | 1.40 | 5.00 |
| 3 - 4 | 4.60 | 17.50 |