ID
139

trans-4-Hydroxy-DL-proline

COMPOUND IDENTIFICATION

Molecular Weight
131.130
Formula
C5H9NO3
Property
Acidic

CHROMATOGRAM

trans-4-Hydroxy-DL-proline

ANALYTICAL REPORT

Column Trade Name
(Column size / Particle size)
CHIRALPAK® ZWIX(-)
(3 x 150 mm 3 µm)
Chromatographic Mode Polar Phase
Mobile Phase Composition 50mM formic acid + 25mM diethylamine in methanol / acetonitrile / water = 49 / 49 / 2
Flow rate (mL/min) 0.5 Temperature (℃) 25
Injection Volume (µL) 0.0015 (mg) Sample Concentration
Detection ELSD
Elution Order D/L
No. Rt K'
1 2.9 0.64
2 4.1 1.29
Alpha Value Resolution
1 - 2 2.02 6.81