COMPOUND IDENTIFICATION
Compound name
- Entecavir Monohydrate
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® IE (4.6 x 250 mm 5 µm) |
|---|---|
| Chromatographic Mode | Normal Phase |
| Mobile Phase Composition | ethanol / methanol / diethylamine = 90 / 10 / 0.1 |
| Flow rate (mL/min) | 0.7 | Temperature (℃) | 25 |
|---|---|---|---|
| Injection Volume (µL) | 0.0025 (mg) | Sample Concentration | |
| Detection | UV-VIS 260 nm | ||
| Elution Order | Enantiomer, API | ||
| No. | Rt | K' |
|---|---|---|
| 1 | 5.8 | 0.36 |
| 2 | 7.7 | 0.79 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 2.21 | 4.23 |