COMPOUND IDENTIFICATION
Compound name
- Hesperidin
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® IE (4.6 x 250 mm 5 µm) |
|---|---|
| Chromatographic Mode | Normal Phase |
| Mobile Phase Composition | methyl tert-butyl ether / methanol = 85 / 15 |
| Flow rate (mL/min) | 1 | Temperature (℃) | 40 |
|---|---|---|---|
| Injection Volume (µL) | 0.02 (mg) | Sample Concentration | |
| Detection | UV-VIS 280 nm | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 6.8 | 1.26 |
| 2 | 8.8 | 1.92 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.53 | 3.88 |