COMPOUND IDENTIFICATION
Compound name
- Tramadol
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® AD-RH (4.6 x 150 mm 5 µm) |
|---|---|
| Chromatographic Mode | Reverse Phase |
| Mobile Phase Composition | 10mM ammonium bicarbonate aq. pH8.5 / acetonitrile = 65 / 35 |
| Flow rate (mL/min) | 1 | Temperature (℃) | 40 |
|---|---|---|---|
| Injection Volume (µL) | 0.005 (mg) | Sample Concentration | |
| Detection | UV-VIS 220 nm | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 5.5 | 2.08 |
| 2 | 6.5 | 2.59 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.25 | 1.52 |