COMPOUND IDENTIFICATION
Compound name
- Levothyroxine
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® ZWIX(-) (3 x 150 mm 3 µm) |
|---|---|
| Chromatographic Mode | Polar Phase |
| Mobile Phase Composition | 50mM formic acid + 25mM diethylamine in methanol / tetrahydrofuran / water = 45 / 50 / 5 |
| Flow rate (mL/min) | 0.5 | Temperature (℃) | 25 |
|---|---|---|---|
| Injection Volume (µL) | 0.002 (mg) | Sample Concentration | |
| Detection | UV-VIS 230 nm | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 6.2 | 3.41 |
| 2 | 7.3 | 4.21 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.23 | 2.53 |