COMPOUND IDENTIFICATION
Compound name
- Phytonadione
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® IF-3 (4.6 x 150 mm 3 µm) |
|---|---|
| Chromatographic Mode | Polar Phase |
| Mobile Phase Composition | 500mM ammonium acetate aq. / acetonitrile / methanol = 2 / 49 / 49 |
| Flow rate (mL/min) | 2.0 | Temperature (℃) | 40 |
|---|---|---|---|
| Injection Volume (µL) | 0.005 (mg) | Sample Concentration | |
| Detection | UV-VIS 254 nm | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 2.1 | 1.61 |
| 2 | 2.7 | 2.31 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.43 | 2.84 |