COMPOUND IDENTIFICATION
Compound name
- DL-Amethopterin
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® ZWIX(+) (4 x 250 mm 3 µm) |
|---|---|
| Chromatographic Mode | Reverse Phase |
| Mobile Phase Composition | methanol / water / formic acid / diethylamine = 800 / 200 / 1.9 / 2.6 |
| Flow rate (mL/min) | 0.5 | Temperature (℃) | 40 |
|---|---|---|---|
| Injection Volume (µL) | Sample Concentration |
0.5 mg/mL in Mobile phase |
|
| Detection | UV-VIS 300 nm | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 8.9 | - |
| 2 | 9.9 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | - | 3.30 |