COMPOUND IDENTIFICATION
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® IM (4.6 x 250 mm 5 µm) |
|---|---|
| Chromatographic Mode | Normal Phase |
| Mobile Phase Composition | n-hexane / ethanol / acetic acid / diethylamine = 80 / 20 / 0.1 / 0.1 |
| Flow rate (mL/min) | 1.0 | Temperature (℃) | 25 |
|---|---|---|---|
| Injection Volume (µL) | 5 | Sample Concentration |
1 mg/mL in Mobile phase |
| Detection | UV-VIS 254 nm | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 13.6 | 3.52 |
| 2 | 15.5 | 4.18 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.19 | 2.81 |