COMPOUND IDENTIFICATION
Compound name
- Serine
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CROWNPAK® CR-I(+) (3 x 150 mm 5 µm) |
|---|---|
| Chromatographic Mode | SFC |
| Mobile Phase Composition | CO2 / methanol / water / trifluoroacetic acid = 70 / 27 / 3 / 0.15 |
| Flow rate (mL/min) | 3.0 | Temperature (℃) | 30 |
|---|---|---|---|
| Injection Volume (µL) | 1 | Sample Concentration | 2.1 mg/mL |
| Detection | LC-MS | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 0.5 | - |
| 2 | 0.8 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | - | 4.22 |
References；
Ultrafast simultaneous chiral analysis of native amino acid enantiomers using supercritical fluid chromatography/tandem mass spectrometry
Journal of Chromatography A 1677 (2022) 463305