COMPOUND IDENTIFICATION
Compound name
- fucose
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® IF-3 (4.6 x 250 mm 3 µm) |
|---|---|
| Chromatographic Mode | Normal Phase |
| Mobile Phase Composition | n-hexane / ethanol / formic acid = 50 / 50 / 0.1 |
| Flow rate (mL/min) | 0.5 | Temperature (℃) | 25 |
|---|---|---|---|
| Injection Volume (µL) | 50 | Sample Concentration |
0.25 mg/mL in Mobile phase |
| Detection | RI | ||
| Elution Order |
第一ピーク：D-fucose (α or βアノマー)
第二ピーク：D-fucose (α or βアノマー) 第三ピーク：L-fucose (α or βアノマー) 第四ピーク：L-fucose (α or βアノマー) |
||
| No. | Rt | K' |
|---|---|---|
| 1 | 10.7 | - |
| 2 | 13.0 | |
| 3 | 19.4 | |
| 4 | 22.7 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | - | 4.71 |
| 2 - 3 | 10.98 | |
| 3 - 4 | 4.72 |
- Comment
- サンプルはMobile phaseに溶解させた後、0.45µm孔径のメンブレンフィルターでろ過した。