COMPOUND IDENTIFICATION
Compound name
- Nadolol (four isomers)
CHROMATOGRAM
ANALYTICAL REPORT
Column Trade Name (Column size / Particle size) |
CHIRALPAK® IA (4.6 x 250 mm 5 µm) |
---|---|
Chromatographic Mode | Normal Phase |
Mobile Phase Composition | n-heptane / ethanol / diethylamine = 85 / 15 / 0.1 |
Flow rate (mL/min) | 1 | Temperature (℃) | 25 |
---|---|---|---|
Injection Volume (µL) | 0.01 (mg) | Sample Concentration | |
Detection | UV-VIS 230 nm | ||
Elution Order |
No. | Rt | K' |
---|---|---|
1 | 10.4 | 2.50 |
2 | 12.1 | 3.00 |
3 | 16.7 | 4.60 |
4 | 27.2 | 8.10 |
Alpha Value | Resolution | |
---|---|---|
1 - 2 | 1.20 | 2.70 |
2 - 3 | 1.50 | 6.30 |
3 - 4 | 1.70 | 8.10 |