ID
138

trans-4-Hydroxy-DL-proline

COMPOUND IDENTIFICATION

Molecular Weight
131.130
Formula
C5H9NO3
Property
Acidic

CHROMATOGRAM

trans-4-Hydroxy-DL-proline

ANALYTICAL REPORT

Column Trade Name
(Column size / Particle size)
CHIRALPAK® ZWIX(+)
(3 x 150 mm 3 µm)
Column Product Page Japanese
Chromatographic Mode Polar Phase
Mobile Phase Composition 50mM formic acid + 25mM diethylamine in methanol / water = 98 / 2
Flow rate (mL/min) 0.5 Temperature (℃) 25
Injection Volume (µL) 0.0015 (mg) Sample Concentration
Detection ELSD
Elution Order L/D
No. Rt K'
1 3.2 0.76
2 4.0 1.20
Alpha Value Resolution
1 - 2 1.58 4.44