ID
140

Tryptophan

COMPOUND IDENTIFICATION

Molecular Weight
204.225
Formula
C11H12N2O2
Property
Amphoteric
Tryptophan

Compound name

Tryptophan

CHROMATOGRAM

Tryptophan

ANALYTICAL REPORT

Column Trade Name
(Column size / Particle size)
CHIRALPAK® ZWIX(+)
(3 x 150 mm 3 µm)
Column Product Page Japanese
Chromatographic Mode Polar Phase
Mobile Phase Composition 50mM formic acid + 25mM diethylamine in methanol / water = 80 / 20
Flow rate (mL/min) 0.5 Temperature (℃) 25
Injection Volume (µL) 0.005 (mg) Sample Concentration
Detection UV-VIS 254 nm
Elution Order D/L
No. Rt K'
1 3.0 0.81
2 3.7 1.25
Alpha Value Resolution
1 - 2 1.53 4.12