COMPOUND IDENTIFICATION
Compound name
- Phytonadione
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® IG-3 (4.6 x 250 mm 3 µm) |
|---|---|
| Chromatographic Mode | Normal Phase |
| Mobile Phase Composition | acetonitrile / ethanol / methanol = 95 / 3 / 2 |
| Flow rate (mL/min) | 1.0 | Temperature (℃) | 25 |
|---|---|---|---|
| Injection Volume (µL) | 0.01 (mg) | Sample Concentration | |
| Detection | UV-VIS 254 nm | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 27.1 | 26.07 |
| 2 | 29.1 | 28.14 |
| 3 | 32.3 | 31.33 |
| 4 | 44.1 | 43.11 |
| 5 | 50.7 | 49.72 |
| 6 | 54.6 | 53.65 |
| 7 | 63.0 | 61.98 |
| 8 | 66.0 | 65.03 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.08 | 2.00 |
| 2 - 3 | 1.11 | 2.00 |
| 3 - 4 | 1.38 | 6.10 |
| 4 - 5 | 1.15 | 3.70 |
| 5 - 6 | 1.08 | 2.00 |
| 6 - 7 | 1.16 | 3.60 |
| 7 - 8 | 1.05 | 1.20 |