COMPOUND IDENTIFICATION
Compound name
- Phytonadione
CHROMATOGRAM
ANALYTICAL REPORT
Column Trade Name (Column size / Particle size) |
CHIRALPAK® IG-3 (4.6 x 250 mm 3 µm) |
---|---|
Chromatographic Mode | Polar Phase |
Mobile Phase Composition | acetonitrile / ethanol / methanol = 95 / 3 / 2 |
Flow rate (mL/min) | 1.0 | Temperature (℃) | 25 |
---|---|---|---|
Injection Volume (µL) | 0.003 (mg) | Sample Concentration | |
Detection | UV-VIS 254 nm | ||
Elution Order |
No. | Rt | K' |
---|---|---|
1 | 27.1 | - |
2 | 29.1 | |
3 | 32.3 | |
4 | 44.1 | |
5 | 50.7 | |
6 | 54.6 | |
7 | 63.0 | |
8 | 66.0 |
Alpha Value | Resolution | |
---|---|---|
1 - 2 | - | 1.20 |
2 - 3 | 2.00 | |
3 - 4 | 2.00 | |
4 - 5 | 2.00 | |
5 - 6 | 3.60 | |
6 - 7 | 3.70 | |
7 - 8 | 6.10 |