COMPOUND IDENTIFICATION
Compound name
- Mannose
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® AD-3 (4.6 x 250 mm 3 µm) |
|---|---|
| Chromatographic Mode | Normal Phase |
| Mobile Phase Composition | n-hexane / ethanol / formic acid = 70 / 30 / 0.1 |
| Flow rate (mL/min) | 0.5 | Temperature (℃) | 25 |
|---|---|---|---|
| Injection Volume (µL) | 50 | Sample Concentration |
0.25 mg/mL in Mobile phase |
| Detection | RI | ||
| Elution Order |
第一ピーク：D-マンノース
第二ピーク：L-マンノース |
||
| No. | Rt | K' |
|---|---|---|
| 1 | 12.9 | - |
| 2 | 14.1 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | - | 2.01 |
- Comment
- サンプルはMobile phaseに溶解させた後、0.45µm孔径のメンブレンフィルターでろ過した。