COMPOUND IDENTIFICATION
Compound name
- cis-4-Hydroxy-DL-proline
CHROMATOGRAM
ANALYTICAL REPORT
Column Trade Name (Column size / Particle size) |
CHIRALPAK® ZWIX(-) (3 x 150 mm 3 µm) |
---|---|
Chromatographic Mode | Polar Phase |
Mobile Phase Composition | 50mM formic acid + 25mM diethylamine in methanol / acetonitrile / water = 49 / 49 / 2 |
Flow rate (mL/min) | 0.5 | Temperature (℃) | 25 |
---|---|---|---|
Injection Volume (µL) | 0.0015 (mg) | Sample Concentration | |
Detection | ELSD | ||
Elution Order | D/L |
No. | Rt | K' |
---|---|---|
1 | 4.9 | 1.74 |
2 | 5.9 | 2.34 |
Alpha Value | Resolution | |
---|---|---|
1 - 2 | 1.34 | 4.29 |