COMPOUND IDENTIFICATION
Compound name
- DL-Amethopterin
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® ZWIX(+) (3 x 150 mm 3 µm) |
|---|---|
| Column Product Page | Japanese |
| Chromatographic Mode | Polar Phase |
| Mobile Phase Composition | 50mM formic acid + 25mM diethylamine in methanol / water = 98 / 2 |
| Flow rate (mL/min) | 0.5 | Temperature (℃) | 45 |
|---|---|---|---|
| Injection Volume (µL) | 0.005 (mg) | Sample Concentration | |
| Detection | UV-VIS 254 nm | ||
| Elution Order | D/L | ||
| No. | Rt | K' |
|---|---|---|
| 1 | 5.3 | 2.32 |
| 2 | 7.6 | 3.74 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.61 | 6.29 |