COMPOUND IDENTIFICATION
Compound name
- Dorzolamide (racemic)
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® AD-3 (4.6 x 250 mm 3 µm) |
|---|---|
| Chromatographic Mode | Normal Phase |
| Mobile Phase Composition | n-hexane / ethanol / diethylamine = 80 / 20 / 0.1 |
| Flow rate (mL/min) | 1 | Temperature (℃) | 40 |
|---|---|---|---|
| Injection Volume (µL) | Sample Concentration | ||
| Detection | UV-VIS 254 nm | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 11.3 | 2.77 |
| 2 | 13.8 | 3.61 |
| 3 | 15.4 | 4.14 |
| 4 | 16.6 | 4.54 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.30 | 4.62 |
| 2 - 3 | 1.15 | 2.64 |
| 3 - 4 | 1.10 | 1.91 |