APPLICATION ID 104

N-3,5-DNB-DL-Leucine

COMPOUND IDENTIFICATION

Compound Name N-3,5-DNB-DL-Leucine
N-3,5-DNB-DL-Leucine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(+)

150 x 3mm / 3µm

Mobile Phase Composition

12.5mM 2-amino-1-butanol + 12.5mM NH4OAc in methanol = 100

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.0025

Temperature (°C)

25

Pressure (MPa)

Detection

UV 270 nm

Elution order

No.

Rt

K’

1

1.22

2

1.61

Alpha Value

Resolution

1 - 2

5.53

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