APPLICATION ID 107

N-alpha-BOC-DL-histidine

COMPOUND IDENTIFICATION

Compound Name N-alpha-BOC-DL-histidine
N-alpha-BOC-DL-histidine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(+)

150 x 3mm / 3µm

Mobile Phase Composition

50mM formic acid + 25mM diethylamine in methanol / acetonitrile / water = 49 / 49 / 2

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.0015

Temperature (°C)

25

Pressure (MPa)

6.5

Detection

UV 230 nm

Elution order

D/L

No.

Rt

K’

1

4.85

1.99

2

5.88

2.61

Alpha Value

Resolution

1 - 2

1.32

3.98

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