APPLICATION ID 1150

Nadolol (four isomers)

COMPOUND IDENTIFICATION

Compound Name Nadolol (four isomers)
Nadolol (four isomers)

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® AD-H

250 x 4.6mm / 5µm

Mobile Phase Composition

acetonitrile / 2-propanol / ethanolamine = 95 / 5 / 0.1

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

1

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

6.7

1.2

2

9.6

2.2

3

12.1

3.1

4

45.5

14.1

Alpha Value

Resolution

1 - 2

1.8

5.6

2 - 3

1.4

5

3 - 4

4.6

17.5

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