APPLICATION ID 1481

Warfarin

COMPOUND IDENTIFICATION

Compound Name Warfarin
Warfarin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IF

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / ethanol / trifluoroacetic acid = 80 / 20 / 0.1

Chromatographic Mode

Normal Phase
Primary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1.0

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

UV 254 nm

Elution order

No.

Rt

K’

1

8.8

1.9

2

11.2

2.7

Alpha Value

Resolution

1 - 2

1.41

4.87

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