COMPOUND IDENTIFICATION
Compound name
- Aliskiren
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® IE-3 (4.6 x 250 mm 3 µm) |
|---|---|
| Chromatographic Mode | Normal Phase |
| Mobile Phase Composition | methyl tert-butyl ether / acetonitrile / 1,4-dioxane / ethylenediamine = 80 / 20 / 1 / 0.05 |
| Flow rate (mL/min) | 1 | Temperature (℃) | 40 |
|---|---|---|---|
| Injection Volume (µL) | 0.05 (mg) | Sample Concentration | |
| Detection | UV-VIS 280 nm | ||
| Elution Order | RSSS Isomer, API, SRRR Isomer, RRRR Isomer | ||
| No. | Rt | K' |
|---|---|---|
| 1 | 18.9 | 5.30 |
| 2 | 21.0 | 6.00 |
| 3 | 25.3 | 7.43 |
| 4 | 28.0 | 8.33 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.13 | 2.00 |
| 2 - 3 | 1.24 | 2.30 |
| 3 - 4 | 1.12 | 4.13 |