APPLICATION ID 1514

Hesperidin

COMPOUND IDENTIFICATION

Compound Name Hesperidin
Hesperidin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IE

250 x 4.6mm / 5µm

Mobile Phase Composition

methyl tert-butyl ether / methanol = 85 / 15

Chromatographic Mode

Normal Phase
Primary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.02

Temperature (°C)

40

Pressure (MPa)

Detection

UV 280 nm

Elution order

No.

Rt

K’

1

6.77

1.26

2

8.75

1.92

Alpha Value

Resolution

1 - 2

1.53

3.88

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