APPLICATION ID 1542

Tramadol

COMPOUND IDENTIFICATION

Compound Name Tramadol
Tramadol

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ID

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / dichloromethane / 2-propanol / diethylamine = 80 / 10 / 10 / 0.1

Chromatographic Mode

Normal Phase
Primary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

UV 270 nm

Elution order

No.

Rt

K’

1

7.68

1.56

2

8.82

1.94

Alpha Value

Resolution

1 - 2

1.24

2.66

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