APPLICATION ID 1552

Besifloxacin

COMPOUND IDENTIFICATION

Compound Name Besifloxacin
Besifloxacin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CROWNPAK® CR-I(-)

150 x 3mm / 5µm

Mobile Phase Composition

perchloric acid aq. pH1.0 / acetonitrile = 60 / 40

Chromatographic Mode

Reverse Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.004

Temperature (°C)

15

Pressure (MPa)

Detection

UV 295 nm

Elution order

Enantiomer, API

No.

Rt

K’

1

19.67

10.44

2

22.35

11.99

Alpha Value

Resolution

1 - 2

1.15

2.17

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