APPLICATION ID 1564

Warfarin

COMPOUND IDENTIFICATION

Compound Name Warfarin
Warfarin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IE

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / 2-propanol / trifluoroacetic acid = 70 / 30 / 0.1

Chromatographic Mode

Normal Phase

Flow rate (ml/min)

1.0

Injection amount (mg)

0.003

Temperature (°C)

25

Pressure (MPa)

Detection

UV 254 nm

Elution order

No.

Rt

K’

1

9.5

2.2

2

12.3

3.1

Alpha Value

Resolution

1 - 2

1.41

5.1

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