APPLICATION ID 1597

Levothyroxine

COMPOUND IDENTIFICATION

Compound Name Levothyroxine
Levothyroxine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(-)

150 x 3mm / 3µm

Mobile Phase Composition

50mM formic acid + 25mM diethylamine in methanol / tetrahydrofuran / water = 45 / 50 / 5

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.002

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

6.18

3.41

2

7.3

4.21

Alpha Value

Resolution

1 - 2

1.23

2.53

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