APPLICATION ID 1605

Phytonadione

COMPOUND IDENTIFICATION

Compound Name Phytonadione
Phytonadione

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IF-3

150 x 4.6mm / 3µm

Mobile Phase Composition

500mM ammonium acetate aq. / acetonitrile / methanol = 2 / 49 / 49

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

2.0

Injection amount (mg)

0.005

Temperature (°C)

40

Pressure (MPa)

Detection

UV 254 nm

Elution order

No.

Rt

K’

1

2.09

1.61

2

2.65

2.31

Alpha Value

Resolution

1 - 2

1.43

2.84

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