APPLICATION ID 1684

Phytonadione

COMPOUND IDENTIFICATION

Compound Name Phytonadione
Phytonadione

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IG-3

250 x 4.6mm / 3µm

Mobile Phase Composition

acetonitrile / ethanol / methanol = 95 / 3 / 2

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

1.0

Injection amount (mg)

0.003

Temperature (°C)

25

Pressure (MPa)

Detection

UV 254 nm

Elution order

No.

Rt

K’

1

27.1

2

29.1

3

32.3

4

44.1

5

50.7

6

54.6

7

63

8

66

Alpha Value

Resolution

1 - 2

1.2

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