APPLICATION ID 1719

Levothyroxine

COMPOUND IDENTIFICATION

Compound Name Levothyroxine
Levothyroxine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(-)

250 x 3mm / 3µm

Mobile Phase Composition

methanol / tetrahydrofuran / water / formic acid / diethylamine = 450 / 500 / 50 / 1.9 / 2.6

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

8

2

10.2

Alpha Value

Resolution

1 - 2

3.91

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