APPLICATION ID 1899

Warfarin

COMPOUND IDENTIFICATION

Compound Name Warfarin
Warfarin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IB N-5

250 x 4.6mm / 5µm

Mobile Phase Composition

H3PO4 aq. pH2.0 / acetonitrile = 90 / 10

Chromatographic Mode

Reverse Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

254

Elution order

No.

Rt

K’

1

21.4

2.6

2

28.1

3.7

Alpha Value

Resolution

1 - 2

1.43

8.03

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