APPLICATION ID 1993

Ofloxacin

COMPOUND IDENTIFICATION

Compound Name Ofloxacin
Ofloxacin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IH

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / ethanol / acetic acid / ethylenediamine = 70 / 30 / 0.1 / 0.1

Chromatographic Mode

Normal Phase

Flow rate (ml/min)

1.0

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

UV 290 nm

Elution order

No.

Rt

K’

1

12.6

3.2

2

17.2

4.7

Alpha Value

Resolution

1 - 2

1.49

4.83

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