APPLICATION ID 211

Mephobarbital

COMPOUND IDENTIFICATION

Compound Name Mephobarbital
Mephobarbital

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IB-3

150 x 4.6mm / 3µm

Mobile Phase Composition

chloroform / methanol = 98 / 2

Chromatographic Mode

Normal Phase
Secondary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

UV 254 nm

Elution order

No.

Rt

K’

1

4.79

1.64

2

5.35

1.95

Alpha Value

Resolution

1 - 2

1.19

3.5

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