APPLICATION ID 215

N-3,5-DNB-DL-Leucine

COMPOUND IDENTIFICATION

Compound Name N-3,5-DNB-DL-Leucine
N-3,5-DNB-DL-Leucine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IA-3

150 x 4.6mm / 3µm

Mobile Phase Composition

n-hexane / ethanol / trifluoroacetic acid = 80 / 20 / 0.1

Chromatographic Mode

Normal Phase
Primary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.0025

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

4.94

1.9

2

6.17

2.62

Alpha Value

Resolution

1 - 2

1.38

5.1

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