APPLICATION ID 254

Tropicamide

COMPOUND IDENTIFICATION

Compound Name Tropicamide
Tropicamide

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IA-3

150 x 4.6mm / 3µm

Mobile Phase Composition

methyl tert-butyl ether / ethanol / ethanolamine = 94 / 6 / 0.1

Chromatographic Mode

Normal Phase
Primary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

UV 254 nm

Elution order

No.

Rt

K’

1

5.34

2.13

2

6.5

2.81

Alpha Value

Resolution

1 - 2

1.32

4.45

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