APPLICATION ID 255

Warfarin

COMPOUND IDENTIFICATION

Compound Name Warfarin
Warfarin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IB-3

150 x 4.6mm / 3µm

Mobile Phase Composition

n-hexane / ethyl acetate / trifluoroacetic acid = 70 / 30 / 0.1

Chromatographic Mode

Normal Phase
Secondary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

UV 270 nm

Elution order

No.

Rt

K’

1

5.72

2.15

2

8.55

3.71

Alpha Value

Resolution

1 - 2

1.73

5.79

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